Movie S1.

Peripheral CX3CR1+ cells engraft permanently in the retina after corneal injury.CX3CR1+/EGFP bone marrow chimera model 16 months after cell transfer. In the control eye, very few blood-derived CX3CR1+/EGFP cells that are present into the retina are localized around major retinal vessels and the optic nerve head. These cells are primarily positioned in the ganglion cell layer and do not migrate deeper into the tissue (left video panel). In contrast, after acute ocular surface injury significant number of peripheral CX3CR1+/EGFP monocytes infiltrate and permanently engraft into the retina. Sixteen months after the injury, peripherally engrafted CX3CR1+/EGFP cells have occupied the three distinct retinal microglia strata and transformed to ramified cells (right video panel). The morphology and arrangement of these cells resembles that of yolk-sac derived retinal microglia.