To view this video please enable JavaScript, and consider upgrading to a web browser that supports HTML5 video Movie S3. Time-lapse video of SFF-frozen (left) and conventionally frozen (right) culturing cells. For both freezing methods, most live cells spread on the bottom and began to migrate within a few hours. Cryoprotectant-free cryopreservation of mammalian cells by superflash freezing Yoshitake Akiyama, Masato Shinose, Hiroki Watanabe, Shigeru Yamada, and Yasunari Kanda PNAS. 2019. 116:7738-7743 DOI: 10.1073/pnas.1808645116 Download Original Video Browse All Videos »