Movie S2.

related to Figure 3. AnnexinV and PI time lapse during LPS/zVAD induced necroptosis Full time course of LPS/5z7 induced pyroptosis in B6 BMDMs as shown in Figure 3G. Images were taken with Cytation3 automated microscope, at 4x magnification. Images stacks are processed with iVision (BioVision) software. AnnexinV and PI signal were extracted with iVision and plotted on the Y and X axis, respectively. Each frame of the movie are images taken at 2 minutes apart, up to 5 hours post stimulation.