Movie S4.

Final reorganization of the globopodium during the middle stage of chamber formation in Amphistegina lessonii. The globopodium still grows due to formation of dense radial reticulopodia. At the same time, the outer lamellipodium quickly engulfs the previous chamber. Start of time-lapse movie: 02:21:08 from the onset of chamber formation; recorded: 00:36:36; real time/video time: 2196/5 = 439 times faster than real time. Merged fluorescent and transmission light (SiR-actin/aF+TL); fluorescent light only (SiR-actin/aF); autofluorescence (aF).