Movie S1.

Single-color movie of ILV movement in Lamp1+ endosomes. Saos-2 cells were transduced with Lamp1-GFP for 18 h using CellLight Reagents (BacMam 2.0). Cells were washed and incubated with CPMP 2R (300 nM) for 30 min and nuclei were stained with Hoechst 33342 (300 nM) for 5 min. Cells were lifted with TrypLE Express and re-plated into microscopy slides. Cells were incubated in media containing YM201636 (800 nM) for 1 hour. The GFP, RhoB, and Hoechst channels corresponding to the first frame of this movie can be found in Fig. S11B.