Movie S1.

Cotton swab touch treatment and harvesting of tissues grown on agar for proteomic analysis. Touch treatment was applied to 3-week-old adult plants grown on agar. Touch was applied for 40 times (1 time/s) with a cotton swab (touch strength, 84 ± 80 mg). The aerial rosette leaves were touched randomly. Liquid nitrogen was poured onto the plants within one second of the touch stopping. Tissues were then stored at –80 ° C for proteomics analysis. One set of tissues (one glass jar of touch and one glass jar of control) was poured with liquid nitrogen and harvested simultaneously.