Scratch wound assay of keratinocytes with WAKMAR1 knockdown. Confluent keratinocyte layers were scratched using the Essen wound maker to generate approximately 800 μm width wounds. The cell culture plates were placed into IncuCyte live cell analysis system and imaged every 2 hours. Video were generated using the IncuCyte software. 1: keratinocyte transfected with GapmeR Ctrl; 2: keratinocyte transfected with GapmeR WAKMAR1-1; 3: keratinocyte transfected with GapmeR WAKMAR1-2.
Dongqing Li, Lara Kular, Manika Vij, Eva K. Herter, Xi Li, Aoxue Wang, Tongbin Chu, Maria-Alexandra Toma, Letian Zhang, Eleni Liapi, Ana Mota, Lennart Blomqvist, Irène Gallais Sérézal, Ola Rollman, Jakob D. Wikstrom, Magda Bienko, David Berglund, Mona Ståhle, Pehr Sommar, Maja Jagodic, and Ning Xu Landén
PNAS. 2019. 116:9443-9452 DOI: 10.1073/pnas.1814097116