Movie S1.

Innervation is absent from the neonatal testis. Animation of images taken with the Zeiss Z1 light sheet microscope of a cleared neonatal XY gonad stained with antibodies against the pan-neuronal marker TUJ1 (red), the gonadal marker GATA4 (cyan) and counterstained with Hoechst nuclear dye (grayscale). The video shows an animation of the optical sections and rotating views of 3D renders of the light sheet Z-stack.