Movie S12.

Prespore and prestalk cells attached to TgrC1ext/WGA-coated microsphere stimulated with a cAMP gradient. Time-lapse movies of slug-stage cells attached to TgrC1ext/WGA-coated microspheres and stimulated with a cAMP gradient (green) formed by a cAMP-filled glass needle. Prespore cells remained polarized and attached to coated-beads (left panels). Prestalk cells detached from the bead and migrated towards the cAMP source (right panels).