Movie S2.

CD45RABC spontaneously segregates from CD2-mediated close contacts formed by CD48⁺ Jurkat T-cells interacting with CD2- and CD45RABC-presenting SLBs. Representative video showing simultaneous rCD2 accumulation and CD45RABC-Halo segregation from stable cell/SLB contacts for CD48⁺ Jurkat T-cells interacting with a rCD2- and CD45RABC-Halo presenting SLB (rCD2:CD45RABC-Halo ratio of 4:1). The video combines raw data for the CD2 channel (i.e. Alexa Fluor 488-tagged CD2 fluorescence, green; left) with a simultaneously-acquired video of the CD45RABC-Halo channel (TMR labelled CD45RABC, red; right). The video plays 10-fold faster than real-time (5 frames per second). Scale bar, 5 μm.