Movie S3.

Imaging Ca²⁺ release for Jurkat T-cells forming CD45-depleted contacts with labeled-CD2 presenting SLBs. Video collage for a CD48+ Jurkat T-cell; the video combines raw data for the CD45 channel (i.e. CD45 labeled with Alexa Fluor 568-tagged Gap 8.3 Fab (red; left) with a video of the Fluo-4 channel (green; right). The video plays 10-fold faster than real-time (5 frames per second). Scale bar, 5 μm.