Movie S6.

The spatial retention of ABD on mRNA-NPs impacts the spatiotemporal dynamics of F-actin assemblies Translation of Cy5-labelled mRNA(GFP-ABD)-NPs (top three panels) and Cy5-labelled mRNA(ABD-GFP)-NPs performed in the absence of magnetic forces (bottom three panels). Left panels: Cy5 observation of mRNA-NPs. Middle panels: Time dependent production and localization of GFP. Right panels: Merge color images. Confocal fluorescence microscopy, Scale bar: 20 μm, one frame every 15 minutes.

Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function

Shunnichi Kashida, Dan Ohtan Wang, Hirohide Saito, and Zoher Gueroui

PNAS. 2019. 116:13346-13351 DOI: 10.1073/pnas.1900310116