Movie S7.
Translation of non-biotinylated mRNA(ABD-GFP) in the presence of unconjugated Cy5-labelled NPs mRNA(ABD-GFP) Left panels: Cy5 observation of nanoparticles. Middle panels: Time dependent production and localization of ABD-GFP. Right panels: Merge color images. Confocal fluorescence microscopy, Scale bar: 20 μm, one frame every 15 minutes.
Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function
PNAS. 2019. 116:13346-13351 DOI: 10.1073/pnas.1900310116