Tilted LC-PolScope time lapse of a tetrameric raft structure in an achiral background membrane of long rods shows that the tetramer is made up of both left- and right-handed rafts. Tilted LC-PolScope allows us to distinguish between left-handed and right-handed rod twist in membranes. Left-handed rafts show a bright upper edge and dark lower edge, while right-handed rafts show a dark upper edge and bright lower edge. The center raft of the tetramer is left-handed while the outer-rafts are right-handed. Scale bar is 2 μm.
Joia M. Miller, Chaitanya Joshi, Prerna Sharma, Arvind Baskaran, Aparna Baskaran, Gregory M. Grason, Michael F. Hagan, and Zvonimir Dogic
PNAS. 2019. 116:15792-15801 DOI: 10.1073/pnas.1900615116