Movie S1.

Time-lapse imaging of labeling and trafficking of IMPACT-derived lipids. HeLa cells were labeled via RT-IMPACT with (S)-oxoTCO–C1 and Tz–BODIPY as described in the legend to Figure 5B, and time-lapse imaging of the IEDDA reaction with Tz–BODIPY was performed by confocal microscopy, with images acquired every 3 s. Tz–BODIPY was added in between the first and second frames of the movie. This movie shows a population of cells, and the top and middle rows of images in Figure 5B show a representative single cell from this movie. Scale bar: 20 μm.