Movie S5.
Polymerization of 7 μM PfAct1 in the presence of 12.5 mM phosphate. Phosphate slows the fast pointed end kinetics. Filaments were visualized with 0.5 μM actin-chromobody (ChromoTek). Frame rate, 120x real time; bar, 5 μm.
Unusual dynamics of the divergent malaria parasite PfAct1 actin filament
PNAS. 2019. 116:20418-20427 DOI: 10.1073/pnas.1906600116