Movie S1.

Liquid-liquid phase separation of hIAPP. A drop of a 129 µM hIAPP reaction, in a 24.81 µl reaction volume, was pipetted in an uncoated glass bottom MatTek dish. The dish was placed within the environmentally controlled stage chamber of a Zeiss LSM 880 Airyscan Confocal Microscope set-up at 25°C. LLPS was visualised over time by acquiring images using ZEN operating software and a 63x oil objective. The AWI (outer edge of the reaction drop) is on the left-hand side of the video. The arrowheads indicate droplets that will undergo fusion with one another.