Movie S12.

hIAPP aggregation spreads into the bulk solution. 4 µM hIAPP was incubated with 9.5±0.4x10⁵ 0.2 µm fluoresbrite carboxylate YG beads in H₂O, in a well of a 96-well plate. The well was imaged, z stacks encompassing the AWI and an area spanning 1417x1417 µm² were collected for 21 at least 18 h. 3D projections were performed in Imaris and visualised with the AWI flat viewed from the side with the bulk solution above.