hIAPP aggregation at the AWI - bIAPP with avidin D fluorescein in H2O. 3.6 µM hIAPP was incubated with 0.4 µM bIAPP-0.08 µM avidin D fluorescein in H2O in a well of a 96-well plate. The well was imaged, z stacks encompassing the AWI and an area spanning 1417x1417 µm2 were collected for at least 18 h. 3D projections were performed in Imaris and visualised with the AWI at the front facing the experimentalist and the bulk solution behind it.