Movie S6.

Time-lapse imaging of Pf4 liquid crystalline droplet encapsulated P. aeruginosa cells (related to Figs. 6 and S10) A video showing time-lapse imaging of Pf4 liquid crystalline droplet encapsulated P. aeruginosa cells grown on agar pads at room temperature. Images were captured at 10 minute intervals over a 90-minute period. Left, A488-labelled Pf4 liquid crystalline droplets, and right, brightfield of P. aeruginosa cells. Video shows that encapsulated cells are metabolically active and able to grow and divide. Pf4 liquid crystalline droplets are able to dynamically re-arrange to accommodate newly divided cells, in line with the deformations seen in Pf4 filaments associated with P. aeruginosa cells in cryo-ET.

Phage liquid crystalline droplets form occlusive sheaths that encapsulate and protect infectious rod-shaped bacteria

Abul K. Tarafder, Andriko von Kügelgen, Adam J. Mellul, Ulrike Schulze, Dirk G. A. L. Aarts, and Tanmay A. M. Bharat

PNAS. 2020. 117:4724-4731 DOI: 10.1073/pnas.1917726117