Movie S1.
3D micro-CT imaging of a distal mineralized region of the tibialis cranialis tendon prepared by protocol 3. The movie starts with cross-sectional images advancing from mineralized to unmineralized portions of the tissue. At 1 min, the movie changes to longitudinal images. Numerous dark black (empty) canals are observed with white (mineralized) circumferential and interstitial regions surrounding them. Dark black and gray areas about black are thought to be regions of the tissue without Spurr embedding resin or only limited in resin infiltration, respectively. The region also shows extensive channels that interconnect canals. Voxel resolution is 4.5 μm3.