Movie S3.

FIB-SEM images (EsB) and 3D reconstruction of a small, fully mineralized distal tendon region in a transverse profile to the longitudinal direction of the tissue prepared by protocol 3. Canaliculi originating from the lacunae of two representative tenocytes (tangerine) course through the ECM of the tendon composed here of mineralized (white) collagen fibril bundles. In 3D rendering, the canaliculi (gold) surround the bundles and clearly form circumferentially about them. Canaliculi from neighboring cells may interconnect.