Movie S1a.

Time-lapse videos shows fluorescence intensity of DAPI-stained nucleoid DNA of E. coli (a) I-CeuI+ or (b) I-CeuI- strains. The former took 645 min to lose fluorescence or at an estimated chromosomal degradation rate of 200 bp/sec. The latter did not decrease in fluorescence intensity. Time-lapse video of an E. coli I-CeuI+ Glycolysis+ strain producing protein. Cells were stained with DAPI, which decreased in fluorescence as SimCells were generated. The chromosome-free SimCells then produced mCherry.