Movie S1B.
Time-lapse videos shows fluorescence intensity of DAPI-stained nucleoid DNA of E. coli (A) I-CeuI+ or (B) I-CeuI- strains. The former took 645 min to lose fluorescence or at an estimated chromosomal degradation rate of 200 bp/sec. The latter did not decrease in fluorescence intensity.
Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology
PNAS. 2020. 117:6752-6761 DOI: 10.1073/pnas.1918859117