Movie S4.

Time-lapse video of an E. coli I-CeuI+ Glycolysis+ strain producing protein. Cells were stained with DAPI, which decreased in fluorescence as SimCells were generated. The chromosome-free SimCells then produced mCherry.

Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology

Catherine Fan, Paul A. Davison, Robert Habgood, Hong Zeng, Christoph M. Decker, Manuela Gesell Salazar, Khemmathin Lueangwattanapong, Helen E. Townley, Aidong Yang, Ian P. Thompson, Hua Ye, Zhanfeng Cui, Frank Schmidt, C. Neil Hunter, and Wei E. Huang

PNAS. 2020. 117:6752-6761 DOI: 10.1073/pnas.1918859117

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