Movie S5.

Time-lapse video of fluorescence produced by unstable mCherry with the ASV variant of the ssrA protease tag (hosted by wildtype E. coli I-CeuI-). After addition of this tag, mCherry-ASV has a half-life time of about 96 min.

Chromosome-free bacterial cells are safe and programmable platforms for synthetic biology

Catherine Fan, Paul A. Davison, Robert Habgood, Hong Zeng, Christoph M. Decker, Manuela Gesell Salazar, Khemmathin Lueangwattanapong, Helen E. Townley, Aidong Yang, Ian P. Thompson, Hua Ye, Zhanfeng Cui, Frank Schmidt, C. Neil Hunter, and Wei E. Huang

PNAS. 2020. 117:6752-6761 DOI: 10.1073/pnas.1918859117

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