Movie S4.

Optogenetically-synchronized STA phase movie without the photothermal artifact correction applied. (Left) The background phase signal averaged from the region in the dashed circle shows a linear decrease during the 1-ms LED illumination and a slow recovery after that, while the phase signal spatially averaged over the soma (considering the polarity) exhibits the distinct waveform of the action potential. (Right) STA phase movie of one neuron synchronized by the optogenetic stimulation without photothermal artifact correction applied. The location of the cell is outlined by the solid line. Scale bar: 20 μm.

High-speed interferometric imaging reveals dynamics of neuronal deformation during the action potential

Tong Ling, Kevin C. Boyle, Valentina Zuckerman, Thomas Flores, Charu Ramakrishnan, Karl Deisseroth, and Daniel Palanker

PNAS. 2020. 117:10278-10285 DOI: 10.1073/pnas.1920039117