Movie S2.

Apilimod inhibits VSV-MeGFP-ZEBOV entry. Maximal Z-projection from four optical sections separated 0.25 μM apart of SVG-A cells gene-edited to express TagRFP-Rab5c imaged by spinning disc confocal microscopy every 3 seconds for 3 min. Cells were infected with VSV-MeGFP-ZEBOV (MOI = 3) in the presence of CHX with or without 5 μM Apilimod and imaged ∼ 6-7 h post infection.

Inhibition of PIKfyve kinase prevents infection by Zaire ebolavirus and SARS-CoV-2

Yuan-Lin Kang, Yi-ying Chou, Paul W. Rothlauf, Zhuoming Liu, Timothy K. Soh, David Cureton, James Brett Case, Rita E. Chen, Michael S. Diamond, Sean P. J. Whelan, and Tom Kirchhausen

PNAS. 2020. DOI: 10.1073/pnas.2007837117