Movie S1.

Corresponds to still frames from the panel in Figure 1A. Live-cell confocal imaging of 8 μm × 8 μm region of a single AML12 mouse hepatocyte cultured under basal growth conditions and treated with BODIPY-FL-C12 and LysoTracker Deep Red to label LDs and lysosomes in the green and magenta channels, respectively. Movie represents 8 min 18 sec of elapsed time with frames captured every 2 sec. Playback is at 20 frames/sec.

Direct lysosome-based autophagy of lipid droplets in hepatocytes

Ryan J. Schulze, Eugene W. Krueger, Shaun G. Weller, Katherine M. Johnson, Carol A. Casey, Micah B. Schott, and Mark A. McNiven

PNAS. 2020. 117:32443-32452 DOI: 10.1073/pnas.2011442117