Movie S2.

Corresponds to still frames from the panel in Figure 1H. Live-cell confocal imaging of 6 μm × 6 μm region of a single AML12 mouse hepatocyte transiently expressing the PLIN2-mRFP1-EGFP reporter showing direct contact of a lysosome (blue) with the surface of an LD and subsequent release, ‘peeling’ away a structure that is RFP⁺. Movie represents 28 min 13 sec of elapsed time with frames captured every 13 sec. Playback is at 20 frames/sec.