Movie S3.
Corresponds to still frames from the panel in Figure 2A. Live-cell confocal imaging of 4 μm × 4 μm region of a single AML12 mouse hepatocyte cultured under basal growth conditions and treated with BODIPY-558/568-C12 and LysoTracker Deep Red to label LDs and lysosomes in the green and magenta channels, respectively. Movie represents 24 min 15 sec of elapsed time with frames captured every 5 sec. Playback is at 20 frames/sec.
Direct lysosome-based autophagy of lipid droplets in hepatocytes
PNAS. 2020. 117:32443-32452 DOI: 10.1073/pnas.2011442117