Movie S3.

Corresponds to still frames from the panel in Figure 2A. Live-cell confocal imaging of 4 μm × 4 μm region of a single AML12 mouse hepatocyte cultured under basal growth conditions and treated with BODIPY-558/568-C12 and LysoTracker Deep Red to label LDs and lysosomes in the green and magenta channels, respectively. Movie represents 24 min 15 sec of elapsed time with frames captured every 5 sec. Playback is at 20 frames/sec.

Direct lysosome-based autophagy of lipid droplets in hepatocytes

Ryan J. Schulze, Eugene W. Krueger, Shaun G. Weller, Katherine M. Johnson, Carol A. Casey, Micah B. Schott, and Mark A. McNiven

PNAS. 2020. 117:32443-32452 DOI: 10.1073/pnas.2011442117