Movie S2.

Three-dimensional rendering in Imaris of an in vivo fluorescent light-sheet micrograph from an 8dpf MTZ- unablated Tg(mpeg1.1:Dendra2;rpe65a:nfsB-eGFP) larva. This is the same larva shown in Fig. 3A and A’ and SI Appendix, Movie S1. The original z-stack has been cropped (Fig. 3A; yellow box; crop dimensions=X:150μm, Y:200μm, Z:100μm; 1μm interval) to show morphology of MΦs/μglia labeled using the endogenous mpeg1.1:Dendra2 (red)⁺ signal (magenta). White labels endogenous eGFP⁺ signal (RPE). Anterior is up; scale bar represents 10μm. Definitions as follows: dpf, days post-fertilization; MΦs/μglia, macrophages/microglia; MTZ, metronidazole; RPE, retinal pigment epithelium.