Movie S1.

Ligand-independent polarization of PGCs. Time-lapse movies of PGC polarization in the absence of the chemokine Cxc12a. Lifeact (magenta) labels the cell front and the rear markers (Ezrin, Esyt2a, MTs and Septin9a) are in green. The movies show the same cells presented in Fig. 1j-l. Frame interval is 8 sec. Time in minutes and seconds. Scale bars 10μm.