Movie S12.

Cell polarization of PGCs with enhanced contractility in the presence of Cxcl12a gradient. Time-lapse movies of PGCs expressing a constitutively active version of the RhoA protein (CARhoA) in the presence of Cxcl12a chemokine gradient (indicated in red below the movies). Lifeact 21 (magenta) labels the cell front and the rear markers (Ezrin, Esyt2a and MTs) are in green. Frame interval is 10 sec. Cells were centered using the Icy software. Time in minutes and seconds. Scale bars 10μm.