Movie S5.

Flow of Extended synaptotagmin 2a (Esyt2a) and the cytoplasmic flow in PGCs. Time-lapse movie of a wild-type PGC where Ypet-Esyt2a and mTomato are labeled. The flow of Esyt2a and the cytoplasmic marker were analyzed using the Bioflow software. Arrows indicate the direction of the flow. The movie shows the same cell presented in Fig. 4A. Frame interval is 500ms. Time in minutes and seconds. Scale bar 10 μm.