Movie S7.

Ligand-independent polarization of PGCs with enhanced contractility. Time-lapse movies of PGCs expressing a constitutively active version of the RhoA protein (CARhoA0 in the absence of the chemokine Cxc12a. Lifeact (magenta) labels the cell front and the rear markers (Ezrin, Esyt2a and MTs (CLIP-170)) are in green. Frame interval is 8 sec. Cells were centered using the Icy software. Time in minutes and seconds. Scale bar 10μm.