Movie S9.

Polarization of PGCs following cell division in response to the chemoattractant Cxcl12a. Time-lapse movies of PGC polarization in the presence of Cxcl12a chemokine gradient (indicated in red below the movies) after cell division. Lifeact (magenta) labels the cell front and the rear markers (Ezrin, Esyt2a and MTs) are in green. The movies show the same cells presented in Fig. 4f-h. Frame interval is 10 sec. Cells were centered using the Icy software. Time in minutes and seconds. Scale bars 10μm.