Morphing between the open structure and closed pore structures depicted in cartoon mode with the four different monomers in different colors, viewed first from the membrane bilayer, and then rotated 90° for a view from the intracellular surface. This was created using the NavMs crystal structure (PDB accession no. 3ZJZ; Bagnéris et al. 2013. Nat. Commun. 4:2465) for the open state and a model of the NavMs closed pore generated based on the NavAb closed structure (PDB accession no. 3RVY; Payandeh et al. 2011. Nature. 475:353–358) using Rosetta software (Leaver-Fay et al. 2011. Methods Enzymol. 487:545–574). In all videos, the morph trajectories were created using the Morph Server (Krebs and Gerstein. 2000. Nucleic Acids Res. 28:1665–1675). Videos of the morphing between the two states were made with PyMOL software (Schrödinger, LLC).
