Movie S1.

Distinct T-cell dynamics during recognition of high- and low-affinity TCR ligands in lymph nodes. Mice received transferred GFP+ OT-I CD8+ T cells, were injected with the high-affinity (N4) or the low-affinity (Q4) peptide, and were subjected to intravital imaging of the popliteal lymph node. Images were acquired every 30 s. Tracks over 5 min are shown in white.