Movie S1.

Conformational rearrangements upon binding ADP•BeF3 in the TerLP74-26 ATPase. An interpolation between TerL apo and ADP•BeF3-bound structures using the UCSF Chimera “Morph Conformations” tool (default parameters) (59) was performed. The movie demonstrates the effects of substrate binding and release on the lid subdomain (pink). A closer view reveals how residues in the P-loop move to engage ADP•BeF3 (Fig. 3C). Finally, a wide view reveals the large-scale movement of the Rossmann fold upon ADP•BeF3 binding and release if the lid is held stationary (Fig. 3D).