Movie S8.

Gradient sensing of gip1Δ cells expressing PHAKT-GFP toward 10 μM cAMP, related to Fig. 5B. A 100-μM cAMP gradient was applied to gip1Δ cells expressing PHAKT-GFP in the presence of 5 μM latrunculin A, and images were taken at 1-s intervals for 130 s (MPEG-4; 1.1 MB). (Scale bar, 10 µm.)