Movie S4.

Ectopic origins of EB3-GFP trajectories in a CAMSAP3-depleted Caco-2 cyst. shRNAi CAMSAP3 Caco-2 cells stably expressing EB3-GFP were cultured for 5 d in Matrigel. The GFP signal is shown. Arrow and arrowheads point to examples of the apical and basal sites, respectively, from which EB3-GFP comets radially emanate. 10-s intervals for 5 min (31 frames).