Movie S3.

Sample clip from courtship movies with aligned male movies after segmentation/alignment. (Left) Two flies (female Canton S wildtype and male PSIΔAB) interacting in a courtship chamber. This movie showcases the wing extension in particular that is indicative of courtship, found by using the behavioral space. (Right) The segmented, scaled, and aligned male used for further analysis. This movie specifically shows the defect in initiating song production in the male PSIΔAB fly.