Movie S6.

Sliding of MTs on a very sparse surface of Cut7FL. Conditions: KPEM100 + 100 mM NaCl gliding buffer, 1 mM ATP. Overlay emphasizes repeated pivoting of the sliding MTs at specific points on the surface. The pivoting behavior suggests that single surface-attached Cut7FL molecules are stepping rapidly and processively toward minus ends. Time code: min and s.