Movie S9.

Cdc42 inhibition and recovery on a 60-kPa Ecad-Fc PA gel. Shown is an MDCK cell stably expressing E-cadherin:dsRed adhered to a 60-kPa Ecad-Fc PA gel. One image was acquired per minute over a 135-min interval 5 h after plating. The first black screen indicates the time at which 20 µM ML141 was added. The second black screen indicates the time at which the inhibitor was washed out. (Scale bar, 10 µm.)