Movie S1.

Live-cell imaging of Rad11 foci in sap1-1 cells through mitosis (example 1). Time-lapse microscopy of a representative cell shows chromosomes during M-phase as revealed by the red RFP-tagged histone, Hht1-RFP. A discrete Rad11-GFP green focus is observed near the fragmented chromatin masses in sap1-1. Cells were imaged on a DeltaVision Elite microscope (GE Healthcare) with a 100× 1.4 NA Plan Super Apochromat oil lens (Olympus) using SoftWoRX 6.0.