Movie S2.

Live-cell imaging of Rad11 foci in sap1-1 cells through mitosis (example 2). Time-lapse microscopy showing chromosomes marked by red RFP-tagged histone, Hht1-RFP, during M-phase. Cells were imaged on a DeltaVision Elite microscope (GE Healthcare) with a 100× 1.4 NA Plan Super Apochromat oil lens (Olympus) using SoftWoRX 6.0.