Movie S4.
TCR–pMHC binding induces CD45 R0 segregation at membrane interfaces. TIRF microscopy of a GUV–SLB interface showing the concentration of 2B4 TCR into microdomains that exclude CD45 R0 upon binding IEk MHC loaded with 2B4_5cc7_2 peptide. Total time elapsed = 7 min.
In vitro reconstitution of T cell receptor-mediated segregation of the CD45 phosphatase
PNAS. 2017. 114:E9338-E9345 DOI: 10.1073/pnas.1710358114