Movie S2.

A grid overlaid with FC40 can survive violent agitation (the movie runs in real time). The grid was printed on a 6 cm dish, 16 different dyes delivered in all pairwise combinations to chambers (see Fig. S1A), the dish placed on a shaker, and the lid of the dish removed. The movie begins when the shaker was started; as the speed of shaking increases, waves form in the FC40 and increase in height. Footprints of chambers remain unchanged throughout. Of course, if chambers made with serum have contact angles of >90° and are closely packed and separated from others by thin fluid walls, shaking can cause neighbors to merge.

Microfluidic chambers using fluid walls for cell biology

Cristian Soitu, Alexander Feuerborn, Ann Na Tan, Henry Walker, Pat A. Walsh, Alfonso A. Castrejón-Pita, Peter R. Cook, and Edmond J. Walsh

PNAS. 2018. 115:E5926-E5933 DOI: 10.1073/pnas.1805449115