Movie S5.

Fluid walls withstand forces exerted by living worms at room temperature (a frame from this movie is illustrated in Fig. 4Ei). An 8 x 8 grid (chambers 2 x 2 mm) was made using S medium + 0.03 mg/ml BSA, bacteria added as food, dishes left at room temperature, worms washed in S medium and transferred to new chambers, and living worms imaged.

Microfluidic chambers using fluid walls for cell biology

Cristian Soitu, Alexander Feuerborn, Ann Na Tan, Henry Walker, Pat A. Walsh, Alfonso A. Castrejón-Pita, Peter R. Cook, and Edmond J. Walsh

PNAS. 2018. 115:E5926-E5933 DOI: 10.1073/pnas.1805449115