Fluid walls withstand forces exerted by living worms at room temperature (a frame from this movie is illustrated in Fig. 4Ei). An 8 x 8 grid (chambers 2 x 2 mm) was made using S medium + 0.03 mg/ml BSA, bacteria added as food, dishes left at room temperature, worms washed in S medium and transferred to new chambers, and living worms imaged.
Cristian Soitu, Alexander Feuerborn, Ann Na Tan, Henry Walker, Pat A. Walsh, Alfonso A. Castrejón-Pita, Peter R. Cook, and Edmond J. Walsh
PNAS. 2018. 115:E5926-E5933 DOI: 10.1073/pnas.1805449115